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Optimized single-nucleus transcriptional profiling by combinatorial indexing

Beth K. Martin, Chengxiang Qiu, Jay Shendure, Eva Nichols, Melissa Phung, Rula Green-Gladden, Sanjay Srivatsan, Ronnie Blecher-Gonen, Brian J. Beliveau, Cole Trapnell, Junyue Cao

Published: 2022-10-18 DOI: 10.1038/s41596-022-00752-0

optimization

single-nucleus transcriptional profiling

combinatorial indexing

sci-RNA-seq

whole-organism analysis

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Supplementary information

Supplementary Information

Supplementary Figs. 1–4 and Supplementary Results

Supplementary Table 1

Indexed primer sets

Supplementary Video 1

Demonstration of nuclei isolation

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Genetic circuit design automation with Cello 2.0

Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

A practical guide to scanning light-field microscopy with digital adaptive optics

Effective high-throughput isolation of fully human antibodies targeting infectious pathogens

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

MOF-derived nanoporous carbons with diverse tunable nanoarchitectures

Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease

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References

Supplementary information

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Optimized single-nucleus transcriptional profiling by combinatorial indexing

Supplementary information

Supplementary Information

Supplementary Table 1

Supplementary Video 1

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