In vitro reconstitution of COPII vesicles fromArabidopsis thalianasuspension-cultured cells

Baiying Li, Yonglun Zeng, Sze Wan Lo, Yusong Guo, Liwen Jiang

Published: 2023-01-03 DOI: 10.1038/s41596-022-00781-9

Arabidopsis thaliana

COPII vesicles

in vitro reconstitution

plant microsomes

vesicle purification

AI 解读

Supplementary information

Reporting Summary

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

查看全部

Sections

Figures

References

Supplementary information

Adolf, F. et al. Proteomic profiling of mammalian COPII and COPI vesicles. Cell Rep. 26, 250–265.e5 (2019).
Fries, E. & Rothman, J. E. Transport of vesicular stomatitis virus glycoprotein in a cell-free extract. Proc. Natl Acad. Sci. USA 77, 3870–3874 (1980).
Beckers, C. J., Keller, D. S. & Balch, W. E. Semi-intact cells permeable to macromolecules: use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex. Cell 50, 523–534 (1987).
Baker, D., Hicke, L., Rexach, M., Schleyer, M. & Schekman, R. Reconstitution of SEC gene product-dependent intercompartmental protein transport. Cell 54, 335–344 (1988).
Hicke, L. & Schekman, R. Yeast Sec23p acts in the cytoplasm to promote protein transport from the endoplasmic reticulum to the Golgi complex in vivo and in vitro. EMBO J. 8, 1677–1684 (1989).
Barlowe, C. et al. COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum. Cell 77, 895–907 (1994).
Bednarek, S. Y. et al. COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast. Cell 83, 1183–1196 (1995).
Matsuoka, K. et al. COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes. Cell 93, 263–275 (1998).
Rowe, T. et al. COPII vesicles derived from mammalian endoplasmic reticulum microsomes recruit COPI. J. Cell Biol. 135, 895–911 (1996).
Aridor, M., Weissman, J., Bannykh, S., Nuoffer, C. & Balch, W. E. Cargo selection by the COPII budding machinery during export from the ER. J. Cell Biol. 141, 61–70 (1998).
Melville, D., Gorur, A. & Schekman, R. Fatty-acid binding protein 5 modulates the SAR1 GTPase cycle and enhances budding of large COPII cargoes. Mol. Biol. Cell 30, 387–399 (2019).
Gorur, A. et al. COPII-coated membranes function as transport carriers of intracellular procollagen I. J. Cell Biol. 216, 1745–1759 (2017).
Chen, X. W. et al. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Elife 2, e00444 (2013).
Merte, J. et al. Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure. Nat. Cell Biol. 12, 41–46 (2010).
Fromme, J. C. et al. The genetic basis of a craniofacial disease provides insight into COPII coat assembly. Dev. Cell 13, 623–634 (2007).
Boyadjiev, S. A. et al. Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking. Nat. Genet. 38, 1192–1197 (2006).
Ma, T. et al. A mechanism for differential sorting of the planar cell polarity proteins Frizzled6 and Vangl2 at the trans-Golgi network. J. Biol. Chem. 293, 8410–8427 (2018).
Huang, Y. et al. An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking. Proc. Natl Acad. Sci. U. S. A. 118, e2101287118 (2021).
Brier, L. W., Zhang, M. & Ge, L. Mechanistically dissecting autophagy: insights from in vitro reconstitution. J. Mol. Biol. 428, 1700–1713 (2016).
Robinson, D. G., Brandizzi, F., Hawes, C. & Nakano, A. Vesicles versus tubes: is endoplasmic reticulum-Golgi transport in plants fundamentally different from other eukaryotes? Plant Physiol. 168, 393–406 (2015).
Chung, K. P., Zeng, Y. & Jiang, L. COPII paralogs in plants: functional redundancy or diversity? Trends Plant Sci. 21, 758–769 (2016).
Pimpl, P. et al. In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell 12, 2219–2236 (2000).
Li, B. et al. A distinct giant coat protein complex II vesicle population in Arabidopsis thaliana. Nat. Plants 7, 1335–1346 (2021).
Yokota, E. et al. Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured BY-2 cells. Plant Physiol. 156, 129–143 (2011).
Miao, Y. & Jiang, L. Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells. Nat. Protoc. 2, 2348–2353 (2007).
Hudson, R. T. & Draper, R. K. Interaction of coatomer with aminoglycoside antibiotics: evidence that coatomer has at least two dilysine binding sites. Mol. Biol. Cell 8, 1901–1910 (1997).
Muniz, M., Morsomme, P. & Riezman, H. Protein sorting upon exit from the endoplasmic reticulum. Cell 104, 313–320 (2001).
Espenshade, P. J., Li, W. P. & Yabe, D. Sterols block binding of COPII proteins to SCAP, thereby controlling SCAP sorting in ER. Proc. Natl Acad. Sci. U. S. A. 99, 11694–11699 (2002).
Adolf, F., Rhiel, M., Reckmann, I. & Wieland, F. T. Sec24C/D-isoform-specific sorting of the preassembled ER-Golgi Q-SNARE complex. Mol. Biol. Cell 27, 2697–2707 (2016).
Xu, D. & Hay, J. C. Reconstitution of COPII vesicle fusion to generate a pre-Golgi intermediate compartment. J. Cell Biol. 167, 997–1003 (2004).
Hobman, T. C., Zhao, B., Chan, H. & Farquhar, M. G. Immunoisolation and characterization of a subdomain of the endoplasmic reticulum that concentrates proteins involved in COPII vesicle biogenesis. Mol. Biol. Cell 9, 1265–1278 (1998).
Drakakaki, G. et al. Isolation and proteomic analysis of the SYP61 compartment reveal its role in exocytic trafficking in Arabidopsis. Cell Res. 22, 413–424 (2012).
Wattelet-Boyer, V. et al. Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat. Commun. 7, 12788 (2016).
Bacia, K. et al. Multibudded tubules formed by COPII on artificial liposomes. Sci. Rep. 1, 17 (2011).
Zanetti, G. et al. The structure of the COPII transport-vesicle coat assembled on membranes. Elife 2, e00951 (2013).
Reynolds, G. D., August, B. & Bednarek, S. Y. Preparation of enriched plant clathrin-coated vesicles by differential and density gradient centrifugation. Methods Mol. Biol. 1209, 163–177 (2014).
Mosesso, N., Blaske, T., Nagel, M. K., Laumann, M. & Isono, E. Preparation of clathrin-coated vesicles from Arabidopsis thaliana seedlings. Front. Plant Sci. 9, 1972 (2018).
Dahhan, D. A. et al. Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components. Plant Cell 34, 2150–2173 (2022).
Wang, J. et al. Protein mobilization in germinating mung bean seeds involves vacuolar sorting receptors and multivesicular bodies. Plant Physiol. 143, 1628–1639 (2007).
Tse, Y. C. et al. Identification of multivesicular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cells. Plant Cell 16, 672–693 (2004).
Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254 (1976).

In vitro reconstitution of COPII vesicles fromArabidopsis thalianasuspension-cultured cells

Supplementary information

Reporting Summary

推荐阅读