Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome

Dylan G. Maghini, Eli L. Moss, Summer E. Vance, Ami S. Bhatt

Published: 2020-12-03 DOI: 10.1038/s41596-020-00424-x

nanopore sequencing

long-read sequencing

DNA extraction

metagenomic assembly

human gut microbiome

AI 解读

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Heterogeneity（异质性）

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Short-read metagenomic sequencing and de novo genome assembly of the human gut microbiome can yield draft bacterial genomes without isolation and culture. However, bacterial genomes assembled from short-read sequencing are often fragmented. Furthermore, t
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ACKNOWLEDGMENTS

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Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome

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