Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip

Hans Clevers, Linda Gijzen, Fjodor A. Yousef Yengej, Frans Schutgens, Marianne K. Vormann, Carola M. E. Ammerlaan, Arnaud Nicolas, Dorota Kurek, Paul Vulto, Maarten B. Rookmaaker, Henriette L. Lanz, Marianne C. Verhaar

Published: 2021-03-04 DOI: 10.1038/s41596-020-00479-w

organ-on-a-chip

microfluidic

kidney tubuloids

renal physiology

in vitro model

AI 解读

Extended

Extended Data Fig. 1 Expression levels of markers of the different nephron segments are stable in tubuloids over multiple passages, except for a decrease in the loop of Henle–specific transporter SLC12A1/NKCC2 .

Normalized expression of SLC4A4 / NBCe1 and SLC5A2 / SGLT2 (proximal tubule), SLC12A1 / NKCC2 (loop of Henle), SLC12A3 / NCC (distal tubule) and SCNN1A / ENaCα (collecting duct principal cells) in tubuloids in passages 4, 7 and 10. Tubuloids from two different donors were analyzed for all genes except SLC4A4 / NBCe1 and SLC5A2 / SGLT2 (both one donor). Expression was normalized to the housekeeping gene RPLP0 . Mean and SEM are shown.

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Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip

Extended

Extended Data Fig. 1 Expression levels of markers of the different nephron segments are stable in tubuloids over multiple passages, except for a decrease in the loop of Henle–specific transporter SLC12A1/NKCC2 .

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