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    NCEM Drop - Inactivation of sample in solution (TM-014)

    sandra.crameri

    Published: 2023-05-13 DOI: 10.17504/protocols.io.5jyl89k57v2w/v2
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    Abstract

    This inactivation procedure is used to inactivate a sample for Biological Transfer out of PC4, proir to Conventional NCEM.

    Steps

    Inactivation

    1.

    Add 50µL 16% volume to a Sarstedt tube with o-ring (STK#:2056-U, Catalog #61226C2).

    2.

    Add 150µL sample to the already in the tube. Leave contact time 0h 10m 0s

    3.

    Adsorb 10µL sample to grid 0h 10m 0s , inspect to ensure sample doesn't dry out.

    4.

    Drain excess sample from grid using fliter paper, leave wet.

    5.

    Stain 0h 1m 0s

    6.

    Drain & dry using filter paper

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