NCEM Drop Agar (TM-014)
sandra.crameri
Published: 2023-05-13 DOI: 10.17504/protocols.io.rm7vzb4n5vx1/v1
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Abstract
drop agar
Steps
HEADER
1.
SAN:
SPEC No:
OPERATOR:
Agar preparation
2.
Make 2Mass / % volume 3mL of the solution into 5mL petri dishes and place in fridge to solidify.
3.
Cut 1 x1 cm squares out of agar with scalpel, lift out onto filter paper
Adsorption & Staining
4.
Place 5µL sample on surface of agar square. Immediately float carbon coated formvar filmed grid face down on the droplet and leave until the droplet diffuses into the agar.
5.
Stain 0h 1m 0s
6.
Drain & dry using filter paper
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