Masson-Fontana Staining
Miquel Vila
Published: 2023-10-17 DOI: 10.17504/protocols.io.ewov1q3opgr2/v1
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Abstract
Masson-Fontana Staining for melanin detection
Steps
1.
Heat paraffin tissue sections at 60ºC for 10min.
2.
Incubate sections in xylene (5 min, 3 times) and in ethanol serial washes (100-95-70%-H2O, 5 min each).
3.
Incubate the sections with ammoniac solution for 40 min at 56ºC. Rinse sections in distilled water.
4.
Incubate the sections with sodium thiosulphate for 2 min. Rinse sections in distilled water.
5.
Finally, counterstain with Kernechtrot for 7 min. Rinse sections in distilled water.
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