Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

Giulia Campostrini, Viviana Meraviglia, Elisa Giacomelli, Ruben W. J. van Helden, Loukia Yiangou, Richard P. Davis, Milena Bellin, Valeria V. Orlova, Christine L. Mummery

Published: 2021-03-25 DOI: 10.1038/s41596-021-00497-2

isogenic microtissues

human pluripotent stem cells

cardiac differentiation

3D tissue models

disease modeling

AI 解读

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Tissue-like structures from human pluripotent stem cells containing multiple cell types are transforming our ability to model and understand human development and disease. Here we describe a protocol to generate cardiomyocytes (CMs), cardiac fibroblasts (
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

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Supplementary Information

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