9 mg H₃BO₃

58.5 mg CuSO₄ *5H₂O

1.95 mg KI (Potassium Iodine)

9 mg MnSO₄

7.6 mg NaMoO₄

822 mg ZnSO₄ * 6 H₂O

139.8 mg FeCl₃ * 6H₂O

in 300 mL ddH2O and filter sterilise

Citation:

Heterokaryosis and Parasexuality in the Fungus Ascochyta Imperfecta Author(s): K. E. Sanderson and A. M. Srb Source: American Journal of Botany , Jan., 1965, Vol. 52, No. 1 (Jan., 1965), pp. 72-81 Published by: Wiley Stable URL: http://www.jstor.org/stable/2439977

100X Salt Solution A

10g Ca(NaO₃)₂* 4 H₂O

100 mL ddH₂O

Autoclave

Citation:

Leach, J., Lang, B. R. & Yoder, O. C. Microbiology 128 , 1719-1729, doi:http://doi.org/10.1099/00221287-128-8-1719 (1982).

100X Salt Solution B

2 g KH₂PO₄* 7 H₂O

1.5g NaCl

100 mL H2O

pH 5.3

Autoclave

Citation:

Leach, J., Lang, B. R. & Yoder, O. C. Microbiology 128 , 1719-1729, doi:http://doi.org/10.1099/00221287-128-8-1719 (1982).

Complete Medium Base

10 g glucose OR xylose (substitute glucose for xylose for CMX medium)

1 g Yeast Extract

0.5 g Acid-hydrolysed Casein

0.5 g Enzyme-hydrolysed casein

20g Agar

10 mL Salt A

10 mL Salt B

Make up to 1000 mL with ddH2O and Autoclave

After autoclaving add:

1 mL sterilised micronutrient solution

Citation:

Leach, J., Lang, B. R. & Yoder, O. C. Microbiology 128 , 1719-1729, doi:http://doi.org/10.1099/00221287-128-8-1719 (1982).

Complete Medium Base

0.5 g glucose OR xylose (substitute glucose for xylose for CMX medium)

20 g Sorbose

1 g Yeast Extract

0.5 g acid hydrolysed casein

0.5 g enzyme hydrolysed casein

20g Agar

10 mL Salt A

10 mL Salt B

Make up to 1000 mL with ddH2O and Autoclave

After autoclaving add:

1 mL sterilised micronutrient solution