A bacterial three-hybrid assay for forward and reverse genetic analysis of RNA–protein interactions

Oliver M. Stockert, Chandra M. Gravel, Katherine E. Berry

Published: 2022-02-22 DOI: 10.1038/s41596-021-00657-4

bacterial three-hybrid assay

RNA-protein interactions

genetic analysis

forward genetics

bacterial cell

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This protocol describes a bacterial three-hybrid (B3H) assay, an invivo system that reports on RNA–nobreakprotein interactions and can be implemented in both forward and reverse genetic experiments. The B3H assay connects the strength of an RNA&#
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- References
References
ACKNOWLEDGMENTS

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A bacterial three-hybrid assay for forward and reverse genetic analysis of RNA–protein interactions

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